PGN - Library Detail

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Library Name:

iag04

Total sequences:

4608

Passed:

4168 (90.45%)

Failed - E.coli contamination:

none

Failed - too ambiguous:

3 (0.07%)

Failed - low complexity:

none

Failed - too short:

437 (9.48%)

Failed - low overall quality:

none

Average Good Sequence Length:

659 bp

Organism:

Ipomopsis aggregata

Tisue:

flower buds, elongating flowers, flowers

Developmental Stage:

Host:

E.coli DH10B

Vector:

pBluescript II SK (+)

Authors:

Tom Juenger, University of Texas at Austin, Section of Integrative Biology

Date:

2006-05-17

Comments:

adaptor none, treatment_conditions A large number of rosette leaves were harvested from a number of F2 individuals grown under greenhouse conditions. Leaves were in varying stages of development and a variety of stresses (drought stress, low temp, high light) were imposed on plants prior to harvest to create a diverse mRNA pool. cultivar F2 individuals from a cross between subspecies Candida and Collina cloning_kit Agencourt library_shortname IPOF2 submission_code ipomopsis cloning_host DH10B E. Coli, T1 phage resistant development_stage rosette leaves organism Ipomopsis aggregata vector pAgen 1 tissue leaf tissue subspecies Candida and Collina rs2 Eco RV rs1 Not I

Combined unigene builds available for this organism:

Build Number	Date Built	Unigene Count
7	2006-12-18	195
6	2006-12-18	168
5	2006-12-18	3410
4	2006-12-15	928
3	2006-09-07	2722
2	2006-05-17	1511
1	2005-08-03	478