Ghy08-17f1-f09: TRIMMED | | | |
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GCACGAGGCAGCAGCCATACTAGAGTACCTGACAGCAGAAGTGCTTGAATTGGCCGGCAATGCAAGCAAAGATCTGAAAG
TGAAGCGTATAACACCGAGACATTTACAGCTTGCTATCAGAGGAGATGAAGAACTCGACACCCTCATAAAAGGAACCATT
GCTGGTGGCGGTGTTATTCCTCACATTCACAAGTCACTCATCAACAAGTCTTCTAAAGAGTAGAAGTGTTAAAACCCTAG
AAGAATGTACGACAAGTTTGATCTTGGTGTGTACCTCTCTAAATGGAAAATCAGTAAAAGCTTTAAAGGATGCGGTAAAT
CTTTGGTTTGAGTTGATTATTTGCATAAAGCTTCATGAATATTTTGTTGTGTATCGTTTTACGTACATTTAAGGGCTGTG
GAATTGTTTGTGTTTGGACGTAGTGCAATGTTCTTANNNNNNNNNNNANNANNNNNNNNNNNNNNNNNNNNNNNNCCCCC
GGGTTTTTTTTTTTTTTTTTTTTTTNNNNNTTTTAAAAAAAAAAAAAA
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Color Codes (below/above quality treshold):
| AAAGAG/AAAGAG | default sequence color |
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| Trace File: | [Download] [View] |
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| Raw Sequence: | [View] |
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| Sequence Entered On: | 2005-03-22 15:26:02 |
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| Quality Evaluation: | failed - too ambiguous |
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| Quality Evaluation Details: | sequence has more than 4% ambiguity after trimming |
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| Genbank Submission: | Not yet submitted |
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| Genbank Confirmation: | Not yet confirmed |
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| Submission Delayed: | Not delayed |
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| Automated Blast Results (top 5): |
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| Sequencing Location: | PS |
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| Chemistry: | terminator |
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| Dye: | big-dye |
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| Machine: | ABI 3700 |
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| Sequence Type: | 5' EST |
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| Organism: | Gerbera hybrida |
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| Tisue: | Pappus bristles |
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| Developmental Stage: | dev. stages 2, 4, 6 and 8\
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| Host: | E. coli |
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| Vector: | Uni-ZAP XR |
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| Authors: | R.Laitinen |
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| Date: | 2005-03-18 |
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| Comments: | Primers: EcoRI, XhoI; cloning_kit: Uni-ZAP XR Gigapack III Gold Cloning Kits (Stratagene, La Jolla, USA); Variety Terra Regina. Pappus bristles. Pappus bristles were collected from all flower types from dev. stages 2, 4, 6 and 8. For stage 2, 4 inflorescences were collected; for stage 4, 4 inflorescences; stage 6, 3 inflorescences and stage 8, 2 inflorescences. Total RNA was isolated from the pooled sample. |
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